ncRNA-Seq of Apis cerana:antenna - SRA

SRX24376463: ncRNA-Seq of Apis cerana:antenna
1 ILLUMINA (Illumina HiSeq 4000) run: 95M spots, 28.5G bases, 8.7Gb downloads

Design: Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturers protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and checked using RNase free agarose gel electrophoresis. After total RNA was extracted, rRNAs were removed to retain mRNAs andncRNAs. The enriched mRNAs and ncRNAs were fragmented into short fragments by using fragmentation buffer and reverse transcribed into cDNA with random primers. Second-strand cDNA were synthesized by DNA polymerase I, RNase H, dNTP (dUTP instead of dTTP) and buffer. Next, the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters. Then UNG (Uracil-N-Glycosylase) was used to digest the second-strand cDNA. The digested products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeqTM 4002.

Submitted by: Yangzhou University

Study: Antenna of Apis cerana sequencing

PRJNA1105047 • SRP504201 • All experiments • All runs

show Abstracthide Abstract

We aimed to characterize differences in the uncapping and removal behaviors of worker bees.

Sample:

SAMN41090643 • SRS21133087 • All experiments • All runs

Organism: Apis cerana

Library:

Name: 2-1

Instrument: Illumina HiSeq 4000

Strategy: ncRNA-Seq